Nkhani

01 Zinthu za mutu woyamwa

Pakali pano, phokoso la pipette pamsika limagwiritsa ntchito pulasitiki ya polypropylene, yotchedwa PP, yomwe ndi mtundu wa pulasitiki wosawoneka bwino wokhala ndi inertia yapamwamba ya mankhwala ndi kutentha kwakukulu.

Komabe, yemweyo ndi polypropylene, padzakhala kusiyana kwakukulu mu khalidwe: mkulu khalidwe nozzle zambiri zopangidwa polypropylene zachilengedwe, ndi otsika mtengo nozzle n'kutheka kuti zobwezerezedwanso polypropylene pulasitiki, amatchedwanso zobwezerezedwanso PP, mu nkhani iyi, tingathe. amangonena kuti chinthu chake chachikulu ndi polypropylene.

02 Kupaka kwa mutu woyamwa

Mphuno ya pipette imayikidwa makamaka m'matumba ndi mabokosi. M'misika yokhwima, mabokosi amawongolera; Ndipo pamsika wathu, matumba ali ofala kwambiri panthawiyi - makamaka chifukwa ndi otchipa.

Zomwe zimatchedwa thumba, ndikuyika mitu yoyamwa m'matumba apulasitiki, thumba lililonse la 500 kapena 1000 (chiwerengero cha mitu yayikulu yoyamwa pa thumba chidzakhala chochepa kwambiri). Makasitomala ambiri amagula matumba pambuyo pa mutu woyamwa, ndiyeno pamanja amayika mutu woyamwa mubokosi loyamwa, ndiyeno gwiritsani ntchito mphika wothirira kwambiri wa nthunzi yotseketsa.

Kuonjezera apo, m'zaka zaposachedwa, pakhala pali mtundu watsopano wa kuyika mutu (mitu 8 kapena 10 ya mbale yoyikidwa munsanja, ikhoza kuikidwa mwamsanga m'bokosi lamutu popanda kukhudza mutu). Kuyamwa kumafuna malo osungiramo ochepa komanso kumachepetsa kugwiritsa ntchito pulasitiki, yomwe ndi yogwirizana ndi chilengedwe.

03 Mtengo wamutu woyamwa

Tiyeni tiyambe ndi malangizo onyamula bwino (1000 pa thumba mu makulidwe a 10μL, 200μL ndi 1000μL). Malangizo a bag amagawidwa m'magulu atatu:
① Lowetsani mutu: okwera mtengo kwambiri ndi Eppendorf, thumba mpaka 400 ~ 500 yuan;
(2) Zogulitsa kunja, zopanga zapakhomo: mtundu woyimira kalasi iyi ndi Axygen, mtengo wake nthawi zambiri ndi 60 ~ 80 yuan, Nozzle ya Axygen pamsika ndiyokwera kwambiri;
(3) Kunyumba kuyamwa mutu: monga jiete kuyamwa mutu, mtengo osiyanasiyana zambiri 130-220 yuan; Mtengo wosiyanasiyana wa nesi woyamwa mutu nthawi zambiri ndi 50 ~ 230 yuan; Beekman biological suction mutu, mtengo wamtengo wapatali nthawi zambiri ndi 30-50 yuan. Nthawi zambiri, mtengo wa malangizo omwe ali m'bokosi ndi nthawi 2-3 kuposa maupangiri okhala ndi matumba, pomwe maupangiri osungidwa ndi 10-20% otsika mtengo kuposa maupangiri abokosi.

04 Kukwanira kwa mutu woyamwa

Kuyenerera kwa malangizo a pipette ndi mfundo yomwe ogwiritsa ntchito akuyang'anitsitsa kwambiri tsopano. Chifukwa chiyani? Chifukwa si nozzles onse angagwiritsidwe ntchito mtundu uliwonse wa pipette ndi osiyanasiyana osiyanasiyana, kotero makasitomala ayenera kulabadira kuyenerera kwa nozzles pogula nozzle.

Titha kumvetsetsa makamaka kusintha kwa mutu woyamwa kuchokera kuzinthu izi:

(1) Matchulidwe a mutu woyamwa: mitundu ina yamtundu wina wa pipette imatha kugwiritsa ntchito mutu wake woyamwa, mutu wina woyamwa sungagwiritsidwe ntchito. Mwachitsanzo, Rainin a multichannel pipette ayenera kugwiritsa ntchito LTS nozzles;

(2) Mlingo wa kusintha kwa pipette: zomwe zimachitika kwambiri ndi kuti pipette ikhoza kugwiritsa ntchito ma pipette osiyanasiyana, koma zotsatira za pipetting pambuyo poika pipettes zosiyana sizofanana. Nthawi zambiri, ma nozzles wamba amagwira ntchito bwino, koma mitundu ina imakhala yabwino

(3) Pipette ndi pipette osiyanasiyana kuti zigwirizane: nthawi zonse, voliyumu ya pipette iyenera kukhala yaikulu kuposa kapena yofanana ndi kuchuluka kwa pipette, monga 200μL pipette ingagwiritsidwe ntchito pazitali za pipette za 20μL, 100μL ndi 200μL;

Enieni atha kufunsa ogwira ntchito athu ogulitsa, kuwonetsetsa kuti makasitomala amatha kugula nozzle yoyenera ~

05 Kuyamwa mutu wokhala ndi zosefera

Mutu woyamwa wokhala ndi fyuluta ndi chinthu chosefera pamwamba pa mutu woyamwa, nthawi zambiri woyera. Zosefera nthawi zambiri zimapangidwa ndi polypropylene, zofanana ndi mawonekedwe a fyuluta ya ndudu.

Chifukwa cha kukhalapo kwa fyuluta, chitsanzo chochotsedwa sichingalowe mkati mwa pipette, motero kuteteza zigawo za pipette kuti zisawonongeke ndi zowonongeka, ndipo chofunika kwambiri, kuonetsetsa kuti palibe kuipitsidwa pakati pa zitsanzo. Chifukwa chake, mutu woyamwa wokhala ndi zosefera ndi chida chofunikira chochotsera zitsanzo zosakhazikika komanso zowononga.