Nkhani

A Petri mbale ndi ochiritsira zasayansi chotengera, wopangidwa ndi lathyathyathya chimbale woboola pakati pansi ndi chivundikiro, makamaka zopangidwa pulasitiki ndi galasi, ndi galasi angagwiritsidwe ntchito zipangizo zomera, tizilombo chikhalidwe ndi nyama selo kutsatira chikhalidwe.Ambiri a pulasitiki ndi disposable, oyenera zasayansi inoculation, streaking, ndi kudzipatula mabakiteriya kulima zipangizo zomera.
Njira/sitepe:
1
Zakudya za Petri nthawi zambiri zimapangidwa ndi sing'anga yolimba ya chikhalidwe cha mbale (ndiko komwe dzina la mbale ya mbale).Kupanga mbale sing'anga ndi kusungunula anaika chosawilitsidwa agar sing'anga ndi madzi ofunda (wosabala), kuchotsa mayeso chubu thonje pulagi, Kudutsa pakamwa pa chubu pa lawi la moto nyali mowa, ndiyeno kutsegula pang'ono chivindikiro cha chosawilitsidwa. chikhalidwe mbale, kotero kuti pakamwa pa chubu choyesera akhoza kupita mwakuya.Imagawidwa mofanana pansi pa mbale ndikufupikitsidwa kuti ipeze chikhalidwe cha mbale.
2
Chifukwa kuberekana, chitukuko ndi kukula kwa mabakiteriya zimagwirizana mwachindunji ndi sing'anga yoperekedwa (zakudya), makamaka pakuwunika ndi kusanthula kachulukidwe, zimakhala ndi tanthauzo lalikulu la kuchuluka kwa zakudya zomwe zimaperekedwa.
3
Kuchuluka kwa zakudya zomwe zimaperekedwa pachikhalidwe cha mabakiteriya, kaya ndi yunifolomu, komanso ngati pansi pa mbale ya petri ndi yosalala ndizofunika kwambiri.Ngati pansi pa mbale ya petri ndi yosagwirizana, kugawidwa kwa sing'anga ya agar kumasiyana malinga ndi ngati pansi pa mbale ya petri ndi yosalala kapena ayi.Kuperekako sikukwanira, komwe kumagwirizana kwambiri ndi kusanthula kwachulukidwe, kotero kuti pansi pa mbale ya petri yochuluka imayenera kukhala yosalala kwambiri chifukwa cha chifukwa.Komabe, kuti mudziwe zambiri (kuwunika mabakiteriya, kukula kwa koloni, kuberekana, ndi zina zotero), zakudya zamtundu wa petri zingagwiritsidwe ntchito.
Kusamalitsa
Pambuyo poyeretsa ndi kupha tizilombo musanagwiritse ntchito, kaya mbale ya petri ndi yoyera kapena ayi imakhala ndi chikoka chachikulu pa ntchito, zomwe zingakhudze pH ya sing'anga.Ngati pali mankhwala ena, amalepheretsa kukula kwa mabakiteriya.