Maikulosikopu ndi chida chowonera chomwe chimagwiritsidwa ntchito kwambiri pophunzitsa, kafukufuku wasayansi ndi zina. Mukamagwiritsa ntchito maikulosikopu, pali "chowonjezera" chaching'ono chomwe Bibuke amasowa, ndiko kuti, galasi lakuphimba. Ndiye tigwiritse ntchito bwanji galasi lakuphimba bwino?
Magalasi ophimba ayenera kutsukidwa musanagwiritse ntchito. Itha kutsukidwa ndi madzi oyera, kenako ndikupukuta pang'onopang'ono ndi yopyapyala kapena nsalu ina yofewa, popanga kutsitsa kwakanthawi, kugwiritsa ntchito koyenera kwa "chivundikiro" sitepe iyi ndikunyamula galasi lophimba ndi tweezers, ndi 45 ° Angle yopendekera pang'onopang'ono chivundikirocho. , kotero kuti mbali imodzi ya kukhudzana koyamba ndi dontho pa Wopanda, ndiyeno pang`onopang`ono kuika lathyathyathya. Cholinga cha izi ndikuletsa thovu kuwonekera pansi pa galasi lophimba. Onetsetsani kuti musalole mpweya kulowa pakati pa chinthu chowonera ndi galasi lakuphimba. Izi zidzakhudza kuwonera.
Chifukwa chake titagwiritsa ntchito galasi lakuphimba, liyenera kutsukidwa munthawi yake ndikupukuta kuti liume kuti likonzekere ntchito yotsatira, galasi lakuphimba litha kugwiritsidwanso ntchito, ngakhale kuti ndi lotsika mtengo, ndipo silikhala nthawi imodzi, nthawi wamba liwu lomwe limagwiritsidwa ntchito. m'nyumba zambiri amatsuka ukhondo muzimutsuka bwino
Ngati bwenzi la kufunika ndi apamwamba, angagwiritse ntchito akupanga makina ochapira kuyeretsa, popanda akupanga makina ochapira, ndi woyera kwambiri, ndiye yachibadwa kuyeretsa ndondomeko pambuyo kutsuka, ndiyeno kuika usiku chromic asidi odzola, ndiye muzimutsuka ndi madzi osungunuka, kuti ndiyothandiza kwambiri komanso yabwino komanso yothandiza.
Chithunzi ndi chaching'ono, ntchito, cholinga chachikulu cha galasi chivundikirocho amapangidwa ndi kuona mapangidwe filimu mawonekedwe, pervious kuti kuwala, zosavuta kuona, kusunga madzi chitsanzo makulidwe yunifolomu wosanjikiza lathyathyathya, cholinga chake ndi bwino kuganizira mu kuti kupanga mkulu kusamvana maikulosikopu, yabwino ntchito capillarity, kupanga kuwonjezera mitundu yonse ya reagents ( banga, asidi ndi mchere njira, etc.) pamene mapangidwe gradient. Panthawi imodzimodziyo, galasi lophimba lingathenso kukhala ndi gawo losunga chithunzithunzi chokhazikika komanso chokhazikika, ndikuteteza chitsanzo ku fumbi ndi kukhudzana mwangozi. Panthawi imodzimodziyo, imapewanso kuwononga mandala a cholingacho pogwira mwangozi chithunzicho pamene lens yofuna ikugwa. Mu ma microscope omizidwa ndi mafuta kapena omizidwa m'madzi, chivundikirocho chimatsetsereka kuti chiteteze kukhudzana pakati pa njira yomiza ndi chitsanzo.
Galasi lophimba likhoza kuikidwa pa slide block kuti asindikize chitsanzo ndikuchedwetsa kutaya madzi m'thupi ndi okosijeni kwa chitsanzocho. Tizilombo tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono titha kukulitsidwa mwachindunji pagalasi lakumbuyo musanayike pa slide, ndipo zitsanzo zitha kukhazikitsidwa pazithunzi osati pa slide.
Zithunzi zapachikuto zimakhala ndi makulidwe osiyanasiyana, utali ndi makulidwe. Nthawi zambiri zimakhala zazikulu kuti zigwirizane ndi slide ya microscope, nthawi zambiri 25 x 75mm kukula kwake. Zithunzi zokulirapo ndi zozungulira nthawi zambiri zimakhala 20 mm m'lifupi kapena kucheperapo. Ma slider amakona anayi mpaka 24 x 60mm alipo kuti agulidwe.
Zithunzi zoyambira zimapezeka kwambiri mu makulidwe angapo, odziwika ndi manambala:
No. 0-0.05 mpaka 0.13 mm wandiweyani
N*o*. * 1-1.13 mpaka 0.16 mm wakuda
N*o*. * 1.5-0.16 mpaka 0.19 mm wakuda
N*o*. * 1.5 H - 0.17 mpaka 0.18 mm wandiweyani
No.2-0.19 mpaka 0.23 mm wandiweyani
No. 3-0.25 mpaka 0,35 mm wandiweyani
No. 4-0.43 mpaka 0,64 mm wandiweyani
Kuchuluka kwa galasi lakuphimba ndikofunika kwambiri pa ma microscopes apamwamba. Zolinga zamoyo zokhala ndi maikulosikopu zachilengedwe zimapangidwira kuti slide yagalasi yakuvundikira ya 1.5 (0.17 mm makulidwe) yokhala ndi chokwera chotchingira chivundikiro cha galasi ku slide. Kugwiritsa ntchito zithunzi zachikuto zomwe zimapatuka kuchokera ku makulidwe omwe akuyembekezeredwa kupangitsa kuti pakhale mawonekedwe ozungulira komanso kuchepa kwa mawonekedwe ndi kulimba kwa zithunzi. Zolinga zapadera zitha kugwiritsidwa ntchito ngati zithunzi zopanda magalasi akuvundikira, kapena zitha kukhala ndi mphete zowongolera zomwe zimalola wogwiritsa ntchito kuzolowera makulidwe a magalasi akuvundikira.
Galasi yovundikira imagwira ntchito yofunika kwambiri pakugwiritsira ntchito microscope. Kodi mukudziwa mfundo zomwe zili pamwambazi ndi mfundo zofunika kuziganizira?
Nthawi yotumiza: Apr-26-2022