Nkhani

Mapaipi amagwiritsidwa ntchito m'ma laboratories kusamutsa mililita yamadzimadzi, kuchokera pa 1 ml mpaka 50 ml. Udzu ukhoza kutayidwa mu pulasitiki wosabala kapena wogwiritsidwanso ntchito mugalasi lotha kupanga autoclavable. Ma pipette onsewa amagwiritsa ntchito pipette kulakalaka ndi kutulutsa zakumwa. Miyeso yosiyana ya pipettes imagwiritsidwa ntchito poyesera zosiyana ndi pipette yomweyo. Mwachitsanzo, ma pipette ndi ofunikira pakusakaniza mayankho a mankhwala kapena kuyimitsidwa kwa selo, kusamutsa zakumwa pakati pa mbiya zosiyanasiyana, kapena plating reagents pa kachulukidwe kosiyana. Malingana ngati kusamala kumaperekedwa kwa kuchuluka kwa madzi omwe amafunidwa ndikuthamangitsidwa, ma pipettes angakhale chida chothandiza mu labotale kuti asamutsire mililita yamadzimadzi molondola.

Mitundu ya pipettes ndi zigawo zikuluzikulu za pipettes

Mapaipi nthawi zambiri amakhala machubu apulasitiki osagwiritsidwa ntchito kamodzi; amathanso kukhala machubu agalasi osinthika, ogwiritsidwanso ntchito.

Ma pipettes onse amagwiritsa ntchito pipette pamene amawombera.

Pipette imathetsa kufunikira kwa ochita kafukufuku ku pipette pakamwa monga kale. Njira yachikale yapaipi imeneyi sivomerezedwa chifukwa ili ndi kuthekera koyambitsa zowopsa za zakumwa zomwe zimayamwa mkamwa.

Mpira wa pipette ndi mtundu wa pipette wolondola kwambiri. Nthawi zambiri amaphatikizidwa ndi pipette ya galasi kuti asamutse kuchuluka kwamadzimadzi.

Mapampu a pipette ndi oyeneranso ma pipette agalasi, omwe amatha kusamutsa kuchuluka kwamadzimadzi. Mapampu a pipette nthawi zambiri amakhala oyenera kugawira madzi okwanira mobwerezabwereza.

Ma pipettes othandizira ndi omwe amapezeka kwambiri. Zili ndi zigawo zingapo zazikulu: pakamwa ndi pamene pipette imayikidwa ndi pamene fyuluta nembanemba imayikidwa, yomwe imateteza mkati mwa pipette wothandizira kuti asaipitsidwe ndi madzi.

Mabatani awiri amatha kuwoneka pa chogwirira cha wothandizira pipette. Pamene batani lapamwamba likanikizidwa, madziwo amafunidwa, ndipo batani lapansi likakanizidwa, madziwo amachotsedwa.

Ma pipette ambiri othandizira amakhalanso ndi chowongolera chamadzimadzi. Mwachitsanzo, ikhoza kukhazikitsidwa kuti itulutse madziwo mopanikizika, kapena ikhoza kukhazikitsidwa kuti itulutse mphamvu yokoka popanda mphamvu yakunja.

Ngakhale ma pipette ena othandizira amabwera ndi chingwe chamagetsi, ambiri amakhala ndi batri.

Ma pipette ena othandizira amabwera ndi choyimilira chomwe chimagwirizana ndi malo ogwiritsira ntchito, zomwe zimathandiza kuti pipette ikhale pambali pake pamene sichikugwiritsidwa ntchito popanda kuchotsa pipette.

Monga tanenera kale, pipette yomweyi ingagwiritse ntchito miyeso yosiyanasiyana ya pipettes malingana ndi voliyumu yomwe iyenera kuponyedwa, kuchokera ku 0,1 milliliters mpaka makumi a milliliters.

Basic ntchito ya pipettes

Choyamba, sankhani kukula kwa pipette kutengera kuchuluka kwa madzi omwe mukufuna kusamutsa. Kenaka tsegulani phukusi kuchokera pamwamba, gwirani gawo lokhalo pamwamba pa chizindikiro cha nkhupakupa, lowetsani nsonga ya pipette, ndikuchotsani phukusi lotsalira.

Kenaka, gwirani pipette ndi dzanja limodzi ndikutsegula chivindikiro cha chidebe chomwe chili ndi madzi omwe mukufuna kuti mukhale nawo. Kusunga pipette yowongoka, yesani pang'onopang'ono batani lapamwamba kuti pang'onopang'ono muyambe kuyesa chitsanzo chanu.

Gwiritsani ntchito mzere womaliza pa khoma la pipette kuti muyese kuchuluka kwa madzi omwe mukufuna kusamutsa. Dziwani kuti voliyumu iyenera kuwerengedwa pansi pa meniscus, osati pamwamba.

Kenako mutulutse madziwo mosamala mumtsuko womwe mwasankha, kusamala kuti nsonga ya pipette isakhudze malo aliwonse osabala.

Gwiritsani ntchito kusamala ndi mphamvu yodekha potulutsa madzi, makamaka mukamagwiritsa ntchito ma pipettes ang'onoang'ono, kuti musawononge wothandizira pipette ndi chitsanzo, kapena kuwononga pipette yothandizira. Kusokoneza pogwiritsira ntchito pipette wothandizira kungakwiyitse anthu ena odziwa zambiri mu labu, omwe angafunikire kutenga pipette kuti akonze. Mukapopera madzi ambiri kapena kutulutsa madzi, kuthamanga kwamadzimadzi kumatha kuonjezedwa ndikukanikiza batani mwamphamvu.

Pomaliza, kumbukirani kutaya bwino udzu mutasamutsa madzi.

Kugwiritsa ntchito

Tsopano popeza mukudziwa momwe mungagwiritsire ntchito pipette, tiyeni tiwone mwatsatanetsatane ntchito zina za labotale.

Chinthu chofunika kwambiri pamene culturing ndi plating maselo ndi yunifolomu kugawa maselo mu njira yomaliza. Kuyimitsidwa kwa ma cell kungasakanizidwe mofatsa komanso mogwira mtima pogwiritsa ntchito pipette, yomwe nthawi yomweyo imasakaniza njira zothetsera mankhwala ndi ma reagents.

Pambuyo pa kudzipatula kapena kukonza ma cell oyesera, ma pipette angagwiritsidwe ntchito kusamutsa ma cell a cell kuti akulitse kapena kuwunika kotsatira.